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Blood/urine ethanol
and acetaldehyde levels were estimated after fixed dose of 43 gms
of ethanol in the form of Whisky, Rum, Vodka, Gin, wine and Beer
in six moderate alcohol users. 43 gms of ethanol was divided into
2 pegs and consumed within 1 hour on day 0 and 15 days after Liv.52
treatment. Blood samples were collected every hour upto six hours
and urine samples at 0-3 hours and 3-6 hours. Blood/urine ethanol
and acetaldehyde were estimated by Gas Chromatography.
Significantly higher
ethanol levels were observed after ingestion of Rum and ethanol
levels were lowest with Beer. Acetaldehyde levels correlated well
with ethanol levels on day 0. Irrespective of type of alcoholic
beverage consumed, Liv.52 treatment for 15 days significantly reduced
blood acetaldehyde and increased urinary excretion four fold. The
possibility of destabilisation of acetaldehyde protein adducts in
liver with Liv.52 treatment could be the probable mechanism in reducing
the blood acetaldehyde levels in moderate alcohol users.
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Table 1: Blood acetaldehyde
levels before and 15 days after Liv.52 treatment in moderate
alcohol users (ug/ml) (Mean ± SE)
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|
N=6
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1 hour
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2 hour
|
3 hour
|
4 hour
|
5 hour
|
6 hour
|
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Day 0
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5.40 ± 0.32
|
5.29 ± 0.25
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4.70 ± 0.20
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4.30 ± 0.21
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3.87 ± 0.24
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3.32 ± 0.21
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|
Day 15
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6.51 ± 0.29
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5.00 ± 0.19
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4.20 ± 0.19
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3.30 ± 0.22*
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2.63 ± 0.20*
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2.05 ± 0.19*
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| *p<0.05
(Unpaired 't' test) |
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Table
2: Urine acetaldehyde excretion before and 15 days after Liv.52
treatment (ug) (Mean ± SE)
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N=6
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0-3
hour
|
3-6
hour
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|
Day
0
|
518.37
± 73.01
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198.41
± 22.39
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Day
15
|
1455.83
± 196.33*
|
1057.83
± 89.82*
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|